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Fig. 5. CD24^high and CD24^low variants of MDA-MB-231 breast carcinoma cells. (A) Phenotype of MDA-MB-231 sub-lines. CD24^high-expressing cells were generated by stable overexpression of CD24 and FACS selection. Both sub-lines were transduced with a retroviral CXCR4-GFP expression construct. The transduced cells were sorted for CXCR4 expression. (B) Migration of MDA-MB-231 CD24^high versus CD24^low breast carcinoma cells (either non-transduced or CXCR4-GFP tranduced) in response to SDF-1. Note the overall elevated level of cell migration in CXCR4-GFP-overexpressing cells (**P $≤$ 0.033; *P $≤$ 0.05; ***P $≤$ 0.02). (C) Migration of MDA-MB-231 CD24^high CXCR4-GFP cells after transfection with CD24-specific or control siRNA. FACS analysis indicates reduced CD24 expression 72 hours after CD24 siRNA transfection compared with the control siRNA (*P $≤$ 0.013).

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