JCS02734 Supplementary Material

Files in this Data Supplement:

• Supplemental Figure 1 -

  Fig. S1. Scavenging peroxynitrite reduces nTyr staining in hippocampal neurons. (a) Mice were either infused with buffer or with the peroxynitrite scavenger FeTMPyP. The infusion was followed by intrahippocampal injection of KA. Mice were sacrificed 5 days later. The excitotoxic injury results in detectable protein tyrosine nitrosylation on degenerating hippocampal neurons on the injected side, which is not observed on the uninjected (b) side. (c) Specificity of the anti-nTyr antibody was evaluated by incubating sections with 1 mg/ml nTyr for one hour prior to primary antibody incubation. The incubation with nTyr prevents staining on hippocampal neurons treated with KA (a,c). (d,e) Infusion of FeTMPyP results in reduction of nTyr staining on hippocampal neurons. Some staining is associated only with the few degenerating neurons that are observed after infusion of FeTMPyP and injection of KA (arrows). n=5 per treatment group.

• Supplemental Figure 2 -

  Fig. S2. The specificity of BBB breakdown was assessed by immunofluorescent staining of the tight junction protein occludin. The KA-injected side of wild-type mice and wild-type mice treated with FeTMPyP showed decreased staining with the anti-occludin antibody, indicating a breakdown in the BBB (n=7).