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Fig. 3. Wild-type C57 mice are protected from KA-induced excitotoxicity by the NOS blocker NMMA. (A,B) Two days prior to injection, wild-type or tPA–/– mice were infused with the indicated concentrations of NMMA. The mice were sacrificed five days after the injection of KA with or without a co-injection of tPA and neuronal survival visualized as described in Fig. 1. Infusion of NMMA followed by injection of PBS as a control had no effect on neuronal viability. (C,D) Quantification of the neurodegeneration was performed using Scion Image beta 4.02 and is presented as percentage of surviving neurons in the total hippocampal region on the ipsilateral (injected) side in comparison with the contralateral (non-injected) side. Statistical analysis was performed using one-way ANOVA followed by a Bonferroni-Dunn test for multiple comparisons. Infusion of 5, 100, 200 and 400 µM NMMA prior to KA injection protected neurons from degeneration. *P<0.05; **P<0.01; ***P<0.001; n.s. indicates no significance between groups. n=6 per concentration of NMMA. The experiment was performed three times and the data pooled for statistical comparison. The arrow in A depicts the site of KA injection.
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