QUICK SEARCH:

[advanced]

	Author:		Keyword(s):

Home Help Feedback Subscriptions Archive Search Table of Contents

	Help viewing high resolution images
	Return to article

(Downloading may take up to 30 seconds.
If the slide opens in your browser, select File -> Save As to save it.)
Click on image to view larger version.

Fig. 3. Immunoelectron microscopy analysis of CD63 association with the chlamydial inclusion. HEp-2 cells were infected with C. trachomatis E for 48 hours and analyzed by cryo immunoelectron microscopy. Infected cells immunolabeled with anti-CD63 antibody (anti-mouse-18 nm colloidal gold) revealed CD63 within the chlamydial inclusion and in compartments adjacent to the inclusion that morphologically resembled MVBs (arrows). CD63-labeling was also evident along the inclusion membrane (upper panel, arrowhead; enlarged in right panel) and in small vesicles within the inclusion lumen (lower panel arrowheads; enlarged in right panel). C, Chlamydia; m; mitochondria. Bar, 0.5 µm. For quantification of anti-CD63-labeling, the density of colloidal-gold particles was determined per cross-sectional area of the compartments indicated (particles/µm²). The density was also expressed as fold over background, based on the average background-labeling in mitochondria and nuclei.

Return to article