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Fig. 6. Pma1-Ub can associate with detergent-resistant membranes. Wild-type cells (F1105=W303; MAT{alpha} ura3-1 leu2-3,112 his3-11 trp1-1 ade2-1 can1-100) bearing pXZ28 and pLH609 were induced to express HA-tagged Pma1 or Pma1-Ub for 2 hours by shifting from raffinose medium to galactose-containing medium. Cell lysates were analyzed for detergent insolubility on Optiprep gradients. Pma1 was analyzed by western blot. Gas1 and ALP were analyzed as Triton-insoluble and soluble markers, respectively. Ubiquitylation of Pma1 does not cause its exclusion from detergent-resistant membranes.





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