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Fig. 3. The SBDS protein is distributed relatively evenly throughout the cytoplasm and pseudopod of D. discoideum amoebae that migrate in buffer, but is enriched in the anterior pseudopods of cells that undergo chemotaxis in a spatial gradient of chemoattractant. Cells carrying GFP-tagged SBDS were imaged with LSCM 1 µm above the substratum. (A,B,C) Examples of the even distribution of SBDS-GFP in cells that migrate in buffer. (D) Images of a single cell migrating in buffer, taken every 10 seconds and 1 µm above the substratum. (E,F,G) Examples of the enrichment of SBDS-GFP in the pseudopods of cells undergoing chemotaxis. (H) Images of a single cell undergoing chemotaxis, taken every 10 seconds and 1 µm above the substratum. Arrows represent the direction of increasing chemoattractant concentration; a, anterior end; u, uropod. Bars, 5 µm (bar in A also represents B, D; bar in E also represents F, H).





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