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Fig. 4. Pixel intensity of line scans made with the LSCM across different parts of the cell. Each data point is the average of the line scan taken as the cell either migrates in buffer or undergoes chemotaxis. (A) Diagram of line scans and color code of different parts of cell body. (B-E) Examples of cells migrating in buffer. (F-I) Examples of cells undergoing chemotaxis in a spatial gradient of attractant. Notice that the average pixel intensity across pseudopods is similar to that of the cell body in buffer, but consistently higher in cells undergoing chemotaxis.





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