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Fig. 4. SOCS1 inhibits STAT1 phosphorylation but elevates phosphorylated (p)MAPK levels in RCS cells. (A) RCS-WT and RCS-S1 cells were treated with 100 ng/ml FGF and 5 µg/ml heparin for different periods of time (as indicated) or with interferon- ${alpha}$ (IFN ${alpha}$ ) after 8 hours in serum-free medium. Total cell lysates were subjected to 10% SDS-PAGE followed by western blotting with anti-pSTAT1 ( ${alpha}$ p-STAT1) and anti-STAT1 ( ${alpha}$ STAT1) antibodies. (B) RCS-WT and RCS-S1 cells were treated with 10 ng/ml FGF and 5 µg/ml heparin for different periods of time (as indicated) after 8 hours in serum-free medium. Total cell lysates were subjected to 10% SDS-PAGE followed by western blotting with anti-Myc ( ${alpha}$ c-Myc), anti-pMAPK ( ${alpha}$ pMAPK) and anti-MAPK ( ${alpha}$ MAPK) antibodies.

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