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Fig. 6. PD decreases OPN level more dramatically in RCS SOCS1 than RCS WT cells. (A) RCS WT and RCS S1 cells were starved for 8 hours in serum-free medium, treated with the MAPKK inhibitor PD98059 (PD) or DMSO for 30 minutes and then treated with or without 20 ng/ml FGF and 5 µg/ml heparin for 15 minutes. Cell lysates were subjected to 10% SDS-PAGE followed by western blotting with anti-pMAPK ({alpha} pMAPK) and anti-MAPK ({alpha} MAPK) antibodies. (B) RCS WT and RCS S1 cells were starved for 8 hours in serum-free medium, treated with PD or DMSO for 30 minutes, and then treated as indicated with different concentration of FGF and heparin for 16 hours. Northern analysis of 10 µg total RNA hybridized with OPN probe. The amount of RNA on the membrane was visualized by Methylene-Blue staining of the 18S ribosomal RNA.





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