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Figure 1


Fig. 1. Distance measurements of bw-centromere signals to determine whether the CIDGFP dot closest to the mRFP dot is the centromere of the second chromosome. (A) Projected image of undifferentiated nuclei anterior to the morphogenetic furrow in the eye imaginal discs. The green dots are CIDGFP and the single purple dot represents the lacO repeats inserted in the bw region bound by mRFP-LacI. The background fluorescence of the unbound mRFP-LacI marks the nucleus. In interphase nuclei, the centromeres of the four paired chromosomes in Drosophila are typically observed as three or four dots. (B) Distance between the tagged locus and the closest CIDGFP dot was computed and divided by the radius of the nucleus. The distribution of distances is displayed as box plots. Box plots are calibrated representations of histograms wherein each horizontal line delimits the 10th, 25th, 50th (median), 75th and 90th percentiles. The numbers within the box plots are the number of nuclei included in the analysis. P-values (Mann-Whitney U-test) are shown above brackets for the respective sets. (C) The distribution is similar to that observed in an earlier study using FISH. Probes specific to the bw locus and the AACAC satellite repeats that make up a small subset of 2Rh were used (Thakar and Csink, 2005). (D) A histogram of mRFP-CIDGFP distances corrected by the radius.





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