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Fig. 2. bw^D association is observed in differentiated embryonic neurons but not undifferentiated neuroblasts. (A) Immunofluorescence with antibody against ELAV, a neuron-specific protein, on embryonic neuroblasts cultured for either 5 hours (left) or 15 hours (right). Although the 5-hour cells do not show much signal for ELAV, those cultured for 15 hours do. The 15-hour cells also tend to form ganglionic clusters and extrude neuronal processes. (B) Single section from a series of z-stack images of embryonic neuroblasts expressing CIDGFP (green) and mRFP-LacI protein bound to the bw-region tagged with lacO repeats (purple). (C) Box plots displaying the 2D distance between the mRFP dot and the closest CIDGFP dot corrected by the nuclear radius from projected images. At least three to four coverslips were imaged with no more than 40 nuclei imaged per coverslip. Change in nuclear organization of the bw^D locus is observed with a significant number of cells showing association between the bw^D locus and centromere only after 15 hours of culturing. (D) Histograms of data shown in C.

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