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Figure 6


Fig. 6. Microtubule destabilizers decrease the step size of centromeres, but do not perturb nuclear organization or chromatin dynamics of bwD. (A) Box plots showing the distribution of distances between bwD locus and the nearest CIDGFP signal corrected by the radius. No change was observed in nuclear organization upon treatment with nocodazole and colchicine, two chemicals that destabilize microtubules, as compared with the untreated control. (B) MSD plot (±1 s.e.m.) comparing the movement of bwD-CIDGFP in movies where images were taken at a single plane of focus every 3 seconds. A large change in chromatin dynamics was not observed for the bwD locus upon treatment. (C) MSD (±1 s.e.m.) plots comparing the movement of centromeres in the presence of microtubule-destabilizing agents. Although the diffusion coefficient for the movement of centromeres was comparable, a significant decrease in the radius of confinement was observed upon treatment. (D) Histograms of the distribution of step sizes of centromeres calculated at {Delta}t=15 seconds. A significant decrease in step size of centromeres was observed in nuclei treated with microtubule destabilizers (P<0.0001).





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