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Fig. 9. Freeze-fracture views of lipid droplet-ER membrane associations after immunogold labeling (18 nm gold) for adipophilin in lipid-laden macrophages. (A,B) In convex fractures of lipid droplets and associated ER membranes, prominent labeling for adipophilin is visible on the P-face of the ER membrane adjacent to the lipid droplet, whereas the partner membrane, seen in E-face view in A is devoid of label. The view of the lipid droplet is the E-face equivalent (eq) of the monolayer; this aspect is devoid of label. (C,D) Concave fractures of the lipid droplet revealing the P-face of its outermost monolayer show prominent adipophilin labeling. The E-face of the ER membrane immediately adjacent to the lipid droplet is unlabeled, whereas the P-face of the partner ER membrane shows substantial labeling. In D, the ER cup associated with the lipid droplet has been cross-fractured. Bars, 0.2 µm.
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