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Figure 2


Fig. 2. Trafficking of GFP-CPS and GFP-Sna3p in wild-type fab1{Delta}, apl2{Delta} and apl4{Delta} cells. (A) Yeast cells expressing UbGFP-CPS were inspected by fluorescence and light microscopy as indicated. In all cells UbGFP-CPS was trafficked to the vacuole lumen, confirming that, like fab1{Delta} cells, apl2{Delta} and apl4{Delta} cells have lost specifically the ability to traffic ubiquitylated cargoes. (B) Yeast cells expressing GFP-Sna3p were inspected by fluorescence and light microscopy as indicated. In all cells GFP-Sna3p was trafficked to the vacuole lumen, demonstrating that trafficking from the TGN to the vacuole by the MVB is intact in apl2{Delta} and apl4{Delta} cells.





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