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Figure 6


Fig. 6. Chitin-ring staining is restored to chs6{Delta} cells by deletion of either AP-1 complex genes, FAB1 or VAC14. Yeast cells were fixed, stained with Calcofluor White and inspected by fluorescence microscopy as described in Materials and Methods. (A) Chitin rings are visible in wild-type, apl2{Delta}, apl4{Delta}, chs6{Delta}, fab1{Delta} and vac14{Delta} cells, but not in chs6{Delta} cells as indicated. (B) Deletion of APL2, APL4, FAB1 or VAC14 in chs6{Delta} cells restores chitin-ring staining, implying that Fab1p and Vac14p are required for AP-1 function. (C) Re-introduction of wild-type genes into chs6{Delta} double mutants restores the chs6{Delta} phenotype; no chitin rings are seen in these strains.





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