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Fig. 2. Cerebellar anatomy and innervation of DCN neurons by Purkinje cells are preserved in L7-pepE mice. (A) Sections of wild-type (a,c,e) and Tg-2 (b,d,f) mice were stained with Cresyl Violet (a,b) or with an anti-calbindin D-28K antibody (c-f). The cerebella of transgenic and control mice are indistinguishable when considering size, foliation, layering of cerebellar cortex, morphology, density and arrangement of PC. Bar, 600 µm (a-d); 100 µm (e,f). (B) Double immunofluorescence with anti-calbindin D-28K (a,b,e,f) and anti-synapsin pepE (G304; c,d,g,h) antibodies in cerebellar sections from wild-type (a,c,e,g) and Tg-2 (b,d,f,h) mice. Synapsin pepE immunoreactivity in the cerebellar cortex (a-d) and DCN (e-h) of both groups has a typical distribution with intense staining of the molecular layer, negative PC somata and intense staining at the granule cell layer and in the DCN. Bar, 100 µm (a-d); 200 µm (e-h). (C) High magnification of wild-type (a) and Tg-2 (b) sections double-immunostained with anti-calbindin D-28K (green) and anti-synaptophysin (red) antibodies. PC terminals appear yellow because of the positive staining for both antibodies. Bar, 10 µm. (D) Number (mean ± s.e.m.) of synaptophysin-positive terminals and calbindin- and synaptophysin-positive terminals (PC terminals) in the DCN was calculated as described in Materials and Methods (n=4 per genoptype). PC nerve terminals represented 49±3.7% (means ± s.e.m.; n=4) and 51±2.7 (means ± s.e.m.; n=4) of total synaptophysin-positive DCN terminals in wild-type and Tg-2 mice, respectively.
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