JCS03200 Supplementary Material

Files in this Data Supplement:

• Supplemental Figure 1 -

  Fig. S1. Summary of the MS/MS analyses of the in vitro phosphorylation of CDC25B by CHK1. The sites identified without ambiguity are indicated (blue). Serine residues at position 229 and 230 are underlined. The sequence coverage (red) was 74%.

• Supplemental Figure 2 -

  Fig. S2. Subcellular localisation of S230-phosphorylated CDC25B during mitosis in U2OS cells. U2OS cells were immunostained with the anti-CDC25B-S230-P (green) and anti-γ-tubulin (red) antibodies and co-stained with DAPI. Cells were selected at interphase or at different stages of mitosis.