spacer gif spacer gif spacer gif spacer gif spacer gif
QUICK SEARCH:   [advanced]


spacer gif
     Home     Help     Feedback     Subscriptions     Archive     Search     Table of Contents    

Right arrow Help viewing high resolution images
Right arrow Return to article
(Downloading may take up to 30 seconds.
If the slide opens in your browser, select File -> Save As to save it.)
Click on image to view larger version.


Figure 3


Fig. 3. In vivo phosphorylation of CDC25B on S230 depends on CHK1. (A) U2OS cells expressing HA-CDC25B were synchronized by double thymidine block and released. Six hours after release, cells were treated or not with UCN-01 for 1 hour, and subjected to western blot analyses using anti-CDC25B-S230-P, anti-CDC25B-S563-P, anti-CDC25B-S353-P, anti-HA, anti-cdc2 and anti-cdc2-Y15-P antibodies. Actin was used as loading control. (B,C) U2OS cells expressing HA-CDC25B were transfected with control (Scr) or CHK1 siRNA, or not transfected. Forty-eight hours post-transfection, cells were subjected to (B) western blot analyses with anti-CDC25B-S230-P, anti-CDC25B-S563-P, anti-CHK1 or anti-HA antibodies, with actin as loading control or (C) immunofluorescence analyses with anti-CDC25B-S230-P (green) and anti-{gamma}-tubulin (red) antibodies and co-stained with DAPI. Magnification, 400x.





Right arrow Return to article