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Figure 4


Fig. 4. Mutation of S230 activates CDC25B. (A) Exponentially growing HeLa cells were transfected with pEYFP-C1 (control vector, VC), wild-type EYFP-CDC25B (WT) or EYFP-CDC25B S230A. The cells were fixed 8 hours or 15 hours after transfection and stained with DAPI. The number of EYFP-positive cells displaying a condensed chromatin aspect was determined. The results presented are the average of two and three independent experiments for each time point (8 hours and 15 hours, respectively). (B) Model. CHK1 negatively regulates the activity of CDC25B through multiple phosphorylation events. S230 and S563 phosphorylations, for which data are presented in this report, are indicated in black and grey, respectively. Additional phosphorylations by other kinases occuring from S-phase until entry into mitosis, result in a global phosphorylation pattern that modulates the activity of CDC25B.





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