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Figure 1


Fig. 1. Weak overexpression of Rheb stimulates TOR activity in adults. (A) Northern analysis of adult flies overexpressing Rheb with hs-GAL4 (hs>Rheb) and da-GAL4 (da>Rheb) shows increased Rheb transcript levels. (B) Western analysis of adults overexpressing Rheb with hs-GAL4 shows increased phosphorylation of T398 of S6K. Flies overexpressing Rheb at low levels develop into adult flies with normal body size and weight (wet) (P=0.3456, NS) (C) at a similar rate as control flies (P=0.3998, NS) (D). Error bars represent the s.d. Statistical comparison (T-test): all P values are based on comparison of the overexpressor with control (hsGAL4/w).





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