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Fig. 3. Ectopic cPAR3 accumulates in punctate clusters at the lateral membrane of NEP cells. (A) 12 hours post-electroporation, cPAR3::GFP accumulates in clusters along the plasma membrane of NEP cells (arrows) and also apically (arrowhead). (B) A single electroporated cell with multiple clusters of cPAR::GFP accumulation along the plasma membrane. (C) At 24 hours, cells with high levels of cPAR3 accumulate at the basal region of the NT (arrow) and ectopic mitotic cells (red, pHistH3-positive) are also present in this region (arrowheads in D). (E) At 48 hours, cPAR3 accumulates in rosette-like structures at the basal part of the NE (square), with punctate clusters in other cells (arrow). (F,F') Detail of a rosette-like group of cells [outlined by the dotted line; enlarged from square in (D)], with cPAR3 accumulating at the center (arrow). (G) The cPAR3-N::GFP mutant still localizes apically (arrowhead), but does not form punctate clusters. L, lumen of the NT. Magnification in (A,D), 40x; in (C,E,G), 63x. Bars in (B) and (F,F'), 10 µm.

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