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Figure 6


Fig. 6. PAR complex formation and cNumb subcellular distribution are affected by the presence of excess cPAR3. (A) Western blot shows that overexpression (OE) produces an 80x increase in of cPAR3 levels. (B) Endogenous cPAR6 and aPKC levels are unaffected when cPAR3 is overexpressed. (C,D) Overexpression of cPAR3 recruits more aPKC molecules to the PAR complex. (E) cPAR6/aPKC complex formation is increased in the presence of excess cPAR3. (F) cLGL1 and cNumb protein levels are unaffected when cPAR3 is overexpressed. (G-G'') At 24 hours post-electroporation, cNumb accumulates at the apical region (arrowhead) and in ectopic punctates (arrow). (H,H') Higher magnification of square in (G'') shows that cNumb colocalizes with ectopic cPAR3 punctates (arrowheads), with cNumb vesicles accumulating at the cell periphery (upper half of the image). In the bottom half of the image, showing non-electroporated cells, cNumb-positive vesicles are uniformly distributed in the cytoplasm. Magnification in (G-G''), 20x. Bars in (H,H'), 6 µm.





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