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Figure 5


Fig. 5. CaMKIV* enhances cardiomyogenesis in P19 cells grown in monolayer. P19 cells were transfected with or without CaMKIV* (1.6 µg). (I) Total RNA was isolated from transfected cells after 1 day of transfection and reverse transcribed. PCR products from the amplification of Nkx2-5, Mef2c, Gata4 and ß-actin were detected using Southern blot analysis. (II) P19 cells were fixed after 4 days of transfection, stained with anti-myosin heavy-chain (MyHC) antibody, and examined by immunofluorescence. The number of MyHC-positive cells under each condition was counted. Error bars represent the standard error (+ s.e.); n=3.





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