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Figure 7


Fig. 7. Overexpression of dominant-negative MEF2 (MEF2C/EnR) enhances the development of cardiac muscle. P19(MEF2C/EnR) and P19(control) cells were differentiated in the presence of DMSO. (I), (A) P19(control) and (B) P19 (MEF2C/EnR) cells were fixed and immunostained on day 6 of differentiation with anti-MyHC antibody to detect cardiomyocytes (magnification 400x). (II) RNA was harvested on days 0, 6 and 9. Northern blots containing 6 µg of total RNA were probed as indicated on the right. (III) RNA from day 6 and 9 were reverse-transcribed and Nkx2-5 PCR products were detected using Southern blot analysis. (IV) 10T1/2 fibroblasts were transfected with and without MyoD and MEF2C/EnR. After 6 days in differentiation medium, MyHC-positive cells were counted and normalized for transfection efficiency with GFP and standardized to MyoD conversion levels. Error bars represent the standard error (+ s.e.); n=3.





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