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Figure 2


Fig. 2. MEF2C/EnR expression in cardiomyoblasts initially enhances Nkx2-5 and MEF2C transcript levels and subsequently downregulates cardiac {alpha}-actin, Gata4, Bmp4, Nkx2-5 and Mef2c transcripts. (A-J) P19(Nkx-MEF2C/EnR) and P19(control) cells were differentiated in the presence of DMSO. 12 µg of total RNA that was harvested on days 0, 2, 3, 4 and 6 were northern blotted and probed as indicated (A-E). Nkx2-5, MEF2C/EnR, Mef2c, and ß-actin expression were detected using RT-PCR (G-I). A 750 bp EcoRI fragment of rabbit 18S cDNA (F) or a 520 bp PCR product of mouse ß-actin (J) were used as loading standards.





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