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Figure 2


Fig. 2. Confirmation that the alleles had been targeted to generate heterozygous or homozygous clones as detailed in Fig. 1. (A) Table showing the sizes of the expected EcoRI restriction fragment sizes for each type of clone as detected by the 5' probe. (B) Southern blot analysis of EcoRI digested genomic DNAs from clones heterozygous and homozygous for the deletion using the 5' probe. The result for 1-D9 appears to show a single band, but there are in fact two restriction fragments running very close together (8.5 and 8.4 kb).





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