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Figure 7


Fig. 7. MURF2, Nbr1 and p62 fail to assemble with titin in homozygous M-band-deficient cells. WT and homozygous targeted cells stained for the titin Z-disk epitope T12 (red) and for Nbr1 p62, and MURF2 (green) under conditions optimal for any nuclear localisation for MURF2, are shown. Association with sarcomeric structures is completely lost for both proteins, which adopt a punctate and diffuse staining pattern. MURF2 and Nbr1 can sometimes be found weakly in the nuclei of some ES cells (arrows), which are stained by DAPI (blue). Bars, 5 µm.





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