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Figure 2


Fig. 2. Phosphorylation of exogenous FGF-1 in FGFR-transfected cells. (A) COS-1 cells and (B) HeLa cells were transfected with FGFRs as indicated, pretreated with [33P]phosphate and incubated for 6 hours with FGF-1 or FGF-1 K132E and with heparin where indicated. FGF-1 was extracted from cell lysates as described in Materials and Methods and then separated by SDS-PAGE and blotted onto Immobilon-P membrane. 33P-phosphorylated FGF-1 was detected by autoradiography (upper panel) and the total cell-associated FGF-1 was detected by anti-FGF-1 antibodies (lower panel).





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