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Figure 4


Fig. 4. Kinetics of uptake and phosphorylation of FGF-1. (A) COS-1 cells transfected with FGFR1 or FGFR4 were labeled with [33P]phosphate and incubated with FGF-1 and heparin for the time indicated. FGF-1 was then extracted from the cell lysates and analyzed as in Fig. 2. The amount of 33P-phosphorylated FGF-1 (upper panel) and total intracellular FGF-1 (detected by anti-FGF-1, lower panel) was quantified and plotted against time as percent of the maximal values (B).





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