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Figure 6


Fig. 6. Analyses of the C-terminal tails of the FGFRs. (A) ClustalW alignments of the C-terminal regions of FGFRs from various species as indicated. Numbering on top refers to the amino acids numbering here used for human FGFR1. Amino acid positions of special importance for FGF-1 translocation are indicated by *, ** and ***. (B-D) Ability of wild type and mutated receptors to translocate FGF-1. COS-1 cells were transfected with FGFR-constructs as indicated. The cells were labeled with [33P]phosphate and incubated with FGF-1 and heparin for 6 hours. FGF-1 was then extracted from the cell lysates and analyzed as in Fig. 2. Upper panels show phosphorylated FGF-1 detected by autoradiography and the lower panels show the total of endocytosed FGF-1, detected by anti-FGF-1.





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