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Figure 5


Fig. 5. Reporter proteins distinguish between the effects of 14-3-3 and COPI binding. (A) Schematic representation of the different reporter membrane proteins employed: monomeric CD4, dimeric CD8 and tetrameric CD4pLI. (B-D) Surface expression in COS1 cells of monomeric CD4 (B), dimeric CD8 (C) and tetrameric CD4pLI (D) fused to different C-terminal tails of Kir6.2 (as indicated below). Data are expressed as a fraction of the surface expression observed for the respective AAA construct and represent the mean ± s.e.m. of (B) eight to nine dishes, (C) nine dishes or (D) nine dishes of COS1 cells. (E) Western blots of transfected COS1 cells show similar expression levels for all constructs tested. Detection was performed using antibodies recognizing CD4 or CD8, as indicated. M indicates molecular mass in kDa.





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