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Figure 3


Fig. 3. Similarities between EDEMs and ER {alpha}-mannosidase I. (A) EDEM1, EDEM2, EDEM 3 and ER {alpha}-mannosidase I (MA1B1) share sequence similarity within a region containing the ({alpha}{alpha})7 barrel catalytic domain of class I mannosidases (family 47GH), shown in yellow. The N- and C-regions of the EDEM proteins beyond this domain share no similarity with MA1B1 or significant sequence similarity with each other. MA1B1 contains a transmembrane region, shown in red, which the EDEM proteins lack. The N-terminus of MA1B1 is cytosolic. (B) Sequence conservation between EDEM proteins and MA1B1. Residues highlighted in yellow on the structure of MA1B1 [based on Vallee et al. (Vallee et al., 2000)] are conserved; note the clustering of conserved residues around the active site. The calcium atom seen in the active site is light blue. (C) Back view of the protein in B. (D) Sequence conservation around the active site of the EDEM proteins and MA1B1. Residues within 6Å of kifunensine (pink) in the structure of MA1B1 with kifunensine bound [based on Vallee et al. (Vallee et al., 2000)] are shown. Residues highlighted in yellow are conserved; two residues whose side chains do not point towards kifunensine are not conserved (grey). The calcium ion is in light blue.





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