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Figure 2


Fig. 2. Gliotactin is distributed with Dlg in a range of TCJs. (A) Gliotactin (green) and Neuroglian (red) have only a small degree of overlap at the TCJ in epidermal cells. The merged image is followed by Neuroglian (red channel) and then Gliotactin (green channel) staining in grayscale. At the TCJ the degree of Neuroglian staining is far less than Gliotactin (arrows). Gliotactin can be seen in the SJ domain though at much lower levels than at the TCJ. (B) Gliotactin (green) and Dlg (red) overlap in the TCJ of epidermal cells. Dlg, like Gliotactin, has a greater degree of labeling at the TCJ (arrows). (C) Coracle (green) and Neuroglian (red) overlap in epidermal cells. The merged image is followed by Coracle (green channel) and Neuroglian (red channel) in grayscale. Both proteins show a distinct morphology with ruffled edges (asterisks) in these cells, perhaps reflecting the pleated nature of these septate junctions. (D) Gliotactin compared with Dlg and Neuroglian at the epidermal cell TCJ. For each set of panels the merged image (Gliotactin, green; Dlg, red; Neuroglian, blue) is followed by: Gliotactin (green) and Dlg (red), Neuroglian (green) and Dlg (red). On the bottom row: Dlg (red channel), Gliotactin (green channel) and Neuroglian (blue channel) are in grayscale to show the individual patterns. Gliotactin and Dlg have a greater degree of staining at the TCJ compared with Neuroglian (arrows). Dlg is distinct in pattern from Neuroglian at the SJ, where Neuroglian has a ruffled morphology (asterisks) compared with Dlg. (E,F) Gliotactin compared with Dlg and Neuroglian at the peripodial cell TCJ. For each set of panels the merged image (Gliotactin, green; Dlg, red; Neuroglian, blue) is followed by: Gliotactin (green) and Dlg (red), Neuroglian (green) and Dlg (red). Dlg (red channel), Gliotactin (green channel) and Neuroglian (blue channel) are in grayscale to show the individual patterns. Gliotactin and Dlg have a greater degree of overlap at the ribbon-like TCJ compared with Neuroglian (compare arrows in panels).





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