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Figure 2


Fig. 2. Centralspindlin localizes to the equatorial cortex just before furrow ingression. (A) Localization of PAV::GFP and RacGAP::GFP fusion proteins in cleaving S2 cells. Cells were fixed and stained to detect GFP (green in the merged panels), DNA (blue in the merged panels) and either RacGAP50C or PAV (red in the top and bottom merged panels, respectively). (B) Selected frames from a time-lapse series showing PAV::GFP dynamics in S2 cells. The white arrowheads indicate PAV::GFP localization at the cell equator. Time is in minutes:seconds relative to anaphase onset. Bars, 10 µm.





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