spacer gif spacer gif spacer gif spacer gif spacer gif
QUICK SEARCH:   [advanced]


spacer gif
     Home     Help     Feedback     Subscriptions     Archive     Search     Table of Contents    

Right arrow Help viewing high resolution images
Right arrow Return to article
(Downloading may take up to 30 seconds.
If the slide opens in your browser, select File -> Save As to save it.)
Click on image to view larger version.


Figure 1


Fig. 1. Drosophila Nup214 is in complex with Nup88 and CRM1. The amounts of CRM1 bound to the Nup214-Nup88 complex vary during fly development. Nup214 antiserum was used to coimmunoprecipitate protein complexes of 0-5, 5-10 and 10-15 hour wild-type embryo extracts. Extracts, unbound fractions and precipitates (IP) were analyzed by labeling the western blots with Nup214, CRM1 and Nup88 antibodies. Lamin serves as a loading control. Total amounts of Nup214, Nup88 and CRM1 appear unchanged in the extracts from different embryonic stages. The amount of co-immunoprecipitated CRM1 relative to Nup214 does not change in 0-5 hour and 5-10 hour embryos whereas three times less CRM1 is brought down in 10-15 hour embryos.





Right arrow Return to article