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Figure 6


Fig. 6. Co-expression of Nup214 and Nup88 in the wild type causes formation of cytoplasmic foci and mislocalizes CRM1. (A) Nup214 and Nup88 (HS214+HS88) were expressed with the hsp70 promoter in wild-type third-instar larvae. Heat-treated wild type (wt) served as a control. All panels show confocal sections of malphigian tube nuclei stained either with Nup88 and Nup214 or with Nup214 and CRM1 antisera. Bar, 5 µm. (B) Overexpression of both nucleoporins affects the localization of GFP-NES. GFP-NES was expressed together with Nup88, Nup214 or Nup214 and Nup88 by heat shock induction of third-instar larvae. The ratios of nuclear:cytoplasmic GFP-NES intensities are illustrated in a log2 graph. Nuclear accumulation of the GFP-NES is increased in cells co-expressing both Nup214 and Nup88 (P<0.0001 by pair-wise t-test). Error bars represent s.e.m.





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