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Figure 2


Fig. 2. DAB2 is released during agonist-induced platelet aggregation in a PKC-dependent manner. (A) Release of DAB2 during agonist-induced platelet aggregation. Washed platelets (1.4x108) were stimulated with the indicated concentration of agonists. The platelet pellet and the suspension medium into which protein is secreted were separated for western blot analysis with anti-p96, anti-PF4 and anti-PKC{alpha} antibodies. The lysates of platelet pellets were also used as a control to demonstrate the presence of PKC{alpha} in the platelet pellet but not the suspension medium. (B) PKC-dependent DAB2 release during platelet aggregation. Washed platelets with or without pretreatment of the pan-PKC inhibitor Ro-31-8220 (20 µM) were stimulated by thrombin (0.1 U/ml) or TPA (1 µg/ml). The suspension medium was collected for western blot analysis with anti-96 (DAB2) and anti-PF4 antibodies. The suspension medium from resting platelets was used as a control (C) for the experiment.





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