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Figure 7


Fig. 7. DAB2 inhibits the interaction between platelet and/or megakaryocytic cells and fibrinogen. (A,B) The RGD motif is involved in DAB2-mediated inhibition of TRAP-stimulated platelet adhesion to fibrinogen. Resting and TRAP-stimulated (10 µM) platelets were added to plates pre-coated with fibrinogen (10 µg) in the absence (control) or presence of soluble GST, DAB2-PTB and DAB2-D66E (100 µg/ml) (A), or DAB2-RGD and DAB2-RGE peptides (100 µg/ml) (B) for 3 hours at 37°C. Platelet adhesion with fibrinogen was quantified in Crystal Violet assays. (C,D) Dose-dependent inhibition of K562 cell adhesion to fibrinogen by DAB2-RGD peptide. Ethanol-treated (E) or TPA-treated (T, 10 ng/ml) K562 cells were added to plates pre-coated with fibrinogen (10 µg) in the presence of the indicated concentration of DAB2-RGD or DAB2-RGE peptides. K562-cell adhesion was observed by inverted microscopy (C, 100x). Cell adhesion of TPA-treated cells was also quantified in Crystal Violet assays (D). Data represent the mean ± s.d. (n=4). **P<0.001 compared with platelets stimulated with TRAP only (A and B) or TPA-treated cells in the absence of DAB2 peptide (D).





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