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Figure 2


Fig. 2. p150Glued localises to centrosomes, spindle and interphase microtubules. S2 cells (A and B) or early Drosophila syncitial embryos (C) were fixed and stained with the affinity-purified anti-p150Glued antibodies (red and lower panels in monochrome), the anti-{alpha}-tubulin antibody (green and middle panels in monochrome) and DAPI (DNA staining in blue). (A) Mitotic cells. (B) Interphase cells: the right column shows a 10x magnified view of the border of a flat S2 cell. Note the punctuate staining along microtubule fibres. a, anaphase; c, cytokinesis; m, metaphase; p, prophase; t, telophase. (D) A mitotic cell treated with colchicine to depolymerise the mitotic spindle was stained for polo kinase (red and upper right panel in monochrome) and p150Glued (green and lower panel on the right in monochrome). The lower left panel shows a 10x magnification view of the kinetochore region boxed in the panel above. Bars, 10 µm (A-C); 1 µm (D).





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