spacer gif spacer gif spacer gif spacer gif spacer gif
QUICK SEARCH:   [advanced]


spacer gif
     Home     Help     Feedback     Subscriptions     Archive     Search     Table of Contents    

Right arrow Help viewing high resolution images
Right arrow Return to article
(Downloading may take up to 30 seconds.
If the slide opens in your browser, select File -> Save As to save it.)
Click on image to view larger version.


Figure 4


Fig. 4. Cyclin B, BubR1 and MEI-S322 behaviour in Glued-depleted anaphases. Control or Glued dsRNA-treated S2 cells were cold fixed in methanol (A and B) and stained for Cyclin B (A, green and lower panels in monochrome) and BubR1 (B, green and lower panels in monochrome), tubulin (red) and DNA (blue). Metaphase and anaphase cells are shown. (A) Cyclin B levels are elevated in metaphase and decrease during early anaphase after chromosome segregation. (B) A pool of BubR1 protein remains at the kinetochores of Glued or control dsRNA-treated cells during metaphase and is released from the kinetochore at anaphase onset in control cells. Note that BubR1 signal remains elevated at the kinetochore of chromatids that have not segregated in Glued-depleted anaphases. (C) MEI-S322 protein releases from the centromeres of p150Glued knockdown anaphase cells. Control (left) or Glued dsRNA-treated (right) cells were stained for MEI-S322 protein (red in bottom panels), {alpha}-tubulin (red in top panels) and DNA (blue). Note that the MEI-S322 antigen is released in early anaphase cells but persists at the centromeres of unsegregated chromatids during asynchronous chromosome segregation. Bar, 10 µm.





Right arrow Return to article