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Figure 5


Fig. 5. Aurora B behaviour in p150Glued-depleted anaphases. Control (A) or Glued dsRNA-treated (B) S2 cells (B) were fixed and stained for aurora B (green and lower panels in monochrome), tubulin (red and monochrome in top right panel 4) and DNA (blue). In control or p150Glued-depleted metaphase cells (panel 1), aurora B is recruited to the centromeres. During anaphase A (A, panel 2), note the disappearance of aurora B from the centromeres and its progressive relocalisation to the central spindle during anaphase B (panel 3) and telophase (panel 4). In p150Glued-depleted anaphase cells (panels 2 and 3), aurora B is detected at the centromeres (white arrowhead) that have not segregated (see also supplementary material Fig. S2) and there is no recruitment to the microtubules. Panel 4 shows a p150Glued-depleted anaphase cell in which aurora B signal was overexposed (lower left panel) to show that aurora B signal is not detected on the few microtubules seen between the two chromatin masses. Bar, 10 µm.





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