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Figure 6


Fig. 6. Polo protein kinase behaviour in p150Glued-depleted anaphases. Wild-type (A,B) and Glued RNAi (C,D) cells during anaphase were fixed and stained for cyclin B (green), polo (red, in panels B and D, and lower panels in monochrome), and {alpha}-tubulin (red in panels A and C, and lower panels in monochrome. In control anaphase (B), polo localises to the kinetochores and on central spindle microtubules. In Glued dsRNA-treated cells (D), polo is detected on the kinetochores but not on the microtubules present between the two chromatid masses. Note also the abnormal cortex contraction in this cell (white line). Bar, 10 µm.





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