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Figure 8


Fig. 8. Following Glued RNAi, centrosomes are not properly attached to spindle poles during spindle formation and microtubule bundling is defective after anaphase. Selected frames from time-lapse series of a control cell (A, see also supplementary material Movie 1) or a Glued dsRNA treated cell (B,C and supplementary material Movies 2 and 3) expressing GFP-tagged {alpha} tubulin during mitosis. (A) Mitosis of a control cell. (B) Mitotic spindle formation following nuclear envelope breakdown until metaphase in a Glued dsRNA-treated cell. Note the poor centrosome connection to the spindle poles. (C) Metaphase-to-anaphase transition in a Glued dsRNA-treated cell. To avoid long light exposure and radiation damage, the acquisition started at metaphase. Note the lack of microtubule bundles during anaphase (60:48). The cell is also subjected to strong cortex contraction but is able to organise a central spindle region and a midbody (115:4). The panels are maximum projections of Z series and note that at time 66:48 the two poles are not in the same focal plane. The time scale (minutes: seconds) is shown at the bottom of each panel. Bars, 10 µm.





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