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Figure 3


Fig. 3. Structural organization of pore-free islands. (A) Accumulation of inner nuclear membrane proteins emerin and lamin A/C in the pore-free islands. Nuclei co-stained for nuclear pores and inner nuclear membrane proteins: emerin (top two rows), lamin A/C (third row) and lap2ß (fourth row). Emerin and lamin A/C are enriched in the pore-free islands of G1 (first and third rows) and telophase (second row) nuclei. Note that lap2ß is almost uniformly distributed on the surface of the G1 nucleus (fourth row). (B) Lamin B is excluded from the pore-free island. Surface image of the G1 nuclei (top panels) and middle section of the telophase nuclei (bottom panels), co-stained with DAPI, nuclear pores and lamin B. Merged images of nuclear pores and lamin B are shown on the right. Note that nuclear pores colocalized well with lamin B both in telophase and G1 nuclei (arrowheads).





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