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Fig. 4. Expression levels of lamin A/C affect the nuclear pore dynamics. (A) Low-magnification image of nuclear pore staining of asynchronous HeLa nuclear surfaces. The nuclear pores in most of the cells show more uniform distributions, compared with those of HeLa S3 cells shown in Fig. 1A and Fig. 3A. HeLa telophase nuclei co-stained for emerin and nuclear pores, demonstrated notable pore-free islands enriched with emerin (`core' regions) (right). (B) A-type lamins are upregulated by 4.25 times in HeLaS3 cells that contain distinct pore-free islands. Expression levels of lamin A/C in HeLaS3 cells (left two lanes) and the HeLa cells (right two lanes) shown in A were examined by western blotting. Cell lysate, equivalent to 0.5x10⁴ (left) or 1x10⁴ (right) cells, was loaded per lane for each cell type and verified by Coomassie Brilliant Blue (CBB) staining (bottom panel).

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