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Figure 3


Fig. 3. Association of 14-3-3{gamma} with GFAP during the cell cycle. (A) Coimmunoprecipitation of GFAP and 14-3-3{gamma} in synchronized astrocytes using GFAP antibody. The primary astrocytes were blocked at G1/S phase using aphidicolin (lane 1). Then the cells were released from the block for the indicated time (lanes 2-5). (B) Immunofluorescent triple staining of astrocytes in different phases of the cell cycle with GFAP (green), 14-3-3{gamma} (red) and DNA (blue). Scale bars, 10 µm.





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