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Figure 6


Fig. 6. Phenotype of glial filaments by overexpression of 14-3-3{gamma}. (A) C6 cells were transfected with DsRed-14-3-3{gamma} or DsRed, and GFAP filaments were visualized by immunostaining with GFAP antibody. (a) Normal glia filaments immunostained with rabbit GFAP antibody. (b-c) pDsRed-N1 vector transfection (c, red) did not affect GFAP filaments (b). (d-i) In DsRed-14-3-3{gamma} overexpressing cells (e,h), endogenous GFAP (d,i) showed an aggregation of dots. Scale bar, 10 µm. (B) Distribution of glial filaments in the population of cells expressing the indicated proteins. A minimum of 100 cells was randomly scored in a blinded manner. Each transfection was repeated three times with similar results.





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