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Figure 7


Fig. 7. Motile properties of GFAP in 14-3-3{gamma} overexpressed glioma C6 cells. (A) Cells transfected with GFP-GFAP were imaged before (a,b), after bleaching (c,d), and during recovery (e-h). Images were taken at the indicated times after the end of the bleach pulse. The indicated area is enlarged in pseudocolour (b,d,e-h) and the fluorescence density is shown on the right. (B) Quantitative analysis of FRAP experiments after bleaching. Cells expressing only GFP-GFAP were detected as the control (a). Two different colocalized forms of GFAP, filaments (b) and dots (c), were both examined in GFP-GFAP and DsRed-14-3-3{gamma} cotransfected cells. (C) The t1/2 of FRAP in the above three groups were calculated. Scale bar, 10 µm. Scale bar in magnified figure, 1 µm.





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