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Figure 1


Fig. 1. Clp1p nucleolar release in cells carrying mutations in FEAR pathway homologs. sid2-250 clp1-GFP cells (A) or sid2-250 clp1-GFP cells carrying the spo12 {Delta} (B), plo1-25 (C), alp7 {Delta} (D) or cut1-205 (E) mutations were grown at 25°C, then synchronized by centrifugal elutriation. Cells were then shifted to 36°C. Samples were fixed every 20 minutes in methanol, stained with DAPI, and scored for number of nuclei and Clp1p-GFP localization. At least 100 cells were scored for each time point. Inset images show Clp1p-GFP signal in cells of each strain with Clp1p-GFP released (*) or not released (#).





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