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Figure 3


Fig. 3. Meiosis and spore formation in clp1 {Delta} cells. (A,B) Diploid cells of the genotype h+/h+ ade6-M210 /ade6M-216 pat1-114 /pat1-114 and h+/h+ ade6-M210 /ade6M-216 pat1-114 /pat1-114 clp1::kanMX6/clp1::kanMX6 were grown to mid-exponential phase and then transferred to minimal medium without ammonium chloride to starve cells in G1. Cells were inoculated into complete medium at 33°C to induce meiosis. Samples were fixed at intervals and the number of nuclei per cell was determined. The key shown in A also applies to B. (C,D) Wild-type h+ and h– cells were mated on minimal medium lacking ammonium chloride. Cells were taken from the mating mixture and the percentage of complete asci (C) and number of nuclei per meiotic cell were determined (D).





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